FIG 2.

Binding of AioF-His tag loaded with As to the aioX-aioB intergenic region analyzed by gel mobility shift assays. A 306-bp DNA fragment encompassing the aioX-aioB intergenic region (A to D) was incubated in the presence of recombinant AioF-His tag-As(III) (A and D) or AioF-His tag-As(V) (B and C). Recombinant AioF-His tag-As(V) (C) and AioF-His tag-As(III) (D) proteins were preincubated with dithionite. (E) SDS-PAGE and Western immunodetection with antibodies against the hexahistidine tag of AioF-His tag-As(III) after dithionite treatment.