FIG 3.

Binding of AioF-His tag free of arsenic to the aioX-aioB intergenic region analyzed by gel mobility shift assays. A 306-bp DNA fragment encompassing the aioX-aioB intergenic region was incubated in the presence of the recombinant AioF-His tag-Sb(III) (A), AioF-His tag-As(III) treated with DTT (B) or EDTA (D), or AioF-His tag-As(V) treated with DTT (C) or EDTA (E). (F) SDS-PAGE and Western immunodetection with antibodies against the hexahistidine tag of AioF-His tag-As(III) after DTT or EDTA treatment.