TABLE 1.

Peptides obtained after trypsin digestion of AioF-His tag-As(III) after reduction by DTT and alkylation by iodoacetamide, without treatment, and after direct alkylation by iodoacetamide

Theoretical mass (Da)	Sequencea	AioF-His tag-As(III) trypsin digestionb
		After reduction by DTT (90.3%)	Without treatment (73.4%)	After direct alkylation by iodoacetamide (89.5%)
1,482,667	MHHELPFSSGADR	+	+	+
1,498,662	MHHELPFSSGADR (oxidation of methionine)	+	−	+
1,638,769	MHHELPFSSGADRR	+	+	−
1,654,763	MHHELPFSSGADRR (oxidation of methionine)	+	+	−
2,712,259	STDEDDVAAVFEAAAELFAALSSPMR	+	+	+
2,728,254	STDEDDVAAVFEAAAELFAALSSPMR (oxidation of methionine)	−	−	+
939,532	LSIVCHLR (SH cysteine)	+	+	+
996,554	LSIVCHLR (carbamidomethyl cysteine)	+	−	+
1,444,673	EQDMNVQQIANR	+	+	+
1,460,668	EQDMNVQQIANR (oxidation of methionine)	+	−	+
1,408,742	IGSSQPNTSLHLR	+	+	+
1,177,657	QLHQIGIVDR	+	+	+
896,435	SGQSVTYR	+	+	+
1,009,49	NTFVADLCK (SH cysteine)	+	−	+
1,066,512	NTFVADLCK (carbamidomethyl cysteine)	−	−	+
1,446,659	IVCPGHHHHHHH (SH cysteine)	−	−	−
1,503,68	IVCPGHHHHHHH (carbamidomethyl cysteine)	+	−	+
Disulfide bridges
1,947,022	NTFVADLCK + LSIVCHLR (S-S cysteine)	−	−	+
2,384,191	LSIVCHLR + IVCPGHHHHHHH (S-S cysteine)	−	−	+
2,454,149	NTFVADLCK + IVCPGHHHHHHH (S-S cysteine)	−	−	+

^aCysteine residues are in bold.

^bThe percent sequence coverage is given in parentheses. +, peptides detected; −, peptides not detected.