FIG 6.
Functional analysis of the paeI gene in B. subtilis CU1065. (A) Paenibacillin sensitivity test results of B. subtilis CU1065 and the recombinants harboring fragments proapnA-paeI and propaeA-paeI. The concentrations of crude paenibacillin were applied with a series of 2-fold dilutions and were not quantified. The concentrations of paenibacillin increased starting from the arrow and moving clockwise. (B) The response as the length of the radii of the inhibition halos versus the corresponding relative amount of paenibacillin as shown in panel A. Two high concentrations of paenibacillin (the 5th and 6th dilutions) were removed. This experiment was independently performed at least 3 times, and the results were consistent.