TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli DH5α | Cloning host | 42 |
| Paenibacillus ATCC 842 | Wild type; paenibacillin producer strain | 28 |
| B. subtilis | ||
| BSn5 | Wild type; subtilomycin producer | 16 |
| B1201 (ΔapnB) | Strain BSn5 derivative; ΔapnB::spc | This study |
| B1202 (ΔapnT) | Strain BSn5 derivative; ΔapnT::kan | This study |
| B1203 (ΔybcL) | Strain BSn5 derivative; ΔybcL::kan | This study |
| B1204 (ΔybdG) | Strain BSn5 derivative; ΔybdG::kan | This study |
| B1205 (ΔapnI) | Strain BSn5 derivative; ΔapnI::kan | This study |
| CU1065 | Strain168 derivative; trpC2 attSPβ | 17 |
| B1301 | CU1065 amyE::(spc subtilomycin gene cluster) | This study |
| B1302 | CU1065 amyE::(spc fragment apnI) | This study |
| B1303 | CU1065 amyE::(spc fragment apnT) | This study |
| B1304 | CU1065 amyE::(spc fragment apnI-apnT) | This study |
| B1305 | CU1065 amyE::(spc fragment proapnA apnI) | This study |
| B1306 | CU1065 amyE::(spc fragment proapnA apnT) | This study |
| B1307 | CU1065 amyE::(spc fragment proapnA apnI-apnT) | This study |
| B1308 | CU1065 amyE::(spc fragment proapnA paeI) | This study |
| B1309 | CU1065 amyE::(spc fragment propaeA paeI) | This study |
| Plasmids | ||
| pDG780 | pBluescript KS+ harboring kan (amp kan) | 43 |
| pMD18T simple | Clone vector for E. coli (amp) | TaKaRa |
| pIC333 | Mini-Tn10 transposons delivery vector harboring spc and erm | 44 |
| pDG1730 | Integration vector harboring amyE::spc (amp erm) | 45 |
| pB1201 | pMD18T harboring mutation of ΔapnB::spc | This study |
| pB1202 | pDG780 harboring mutation of ΔapnT::kan | This study |
| pB1203 | pDG780 harboring mutation of ΔybcL::kan | This study |
| pB1204 | pDG780 harboring mutation of ΔybdG::kan | This study |
| pB1205 | pDG780 harboring mutation of ΔapnI::kan | This study |
| pB1301 | pDG1730 harboring amyE::(spc apn gene cluster) | This study |
| pB1302 | pDG1730 harboring amyE::(spc apnI) | This study |
| pB1303 | pDG1730 harboring amyE::(spc apnT) | This study |
| pB1304 | pDG1730 harboring amyE::(spc apnI-apnT) | This study |
| pB1305 | pDG1730 harboring amyE::(spc proapnA apnI) | This study |
| pB1306 | pDG1730 harboring amyE::(spc proapnA apnT) | This study |
| pB1307 | pDG1730 harboring amyE::(spc proapnA apnI-T) | This study |
| pB1308 | pDG1730 harboring amyE::(spc proapnA paeI) | This study |
| pB1309 | pDG1730 harboring amyE::(spc propaeA paeI) | This study |