TABLE 2.
Primer sequences used in this study
| Primer | Sequence (5′→3′)a | Use |
|---|---|---|
| ybcL UpF | CTAACTAGTCAGAGATCAGTAAGTCC | ybcL gene disruption |
| ybcL UpR | TGTCTGCAGAGGTTCCAACTAAAAAGC | ybcL gene disruption |
| ybcL DownF | GGACTCGAGAGTTTGGTTGGATCAAAG | ybcL gene disruption |
| ybcL DownR | TGAGGTACCCTCAGTAATCTTATTAGCC | ybcL gene disruption |
| ApnBUpF | TTTGCATGCTTAGTATTCTCGAGCAG | apnB gene disruption |
| ApnBDownR | TGCGGATCCATTAAATAGGGATAGTG | apnB gene disruption |
| ApnBUpR | CCTTCGTATCCTATTACTCGCATTT | apnB gene disruption |
| ApnBDownF | AGAATCTAGAGCAATCACCTATTCTAAGAGC | apnB gene disruption |
| SpcF | AGTATCGATGCGGTGCTACAGAGTTCTTG | Amplification of spectinomycin resistance gene |
| SpcR | GGGTCTAGAGTAAACGCTGAATATCGTGTT | Amplification of spectinomycin resistance gene |
| ApnTUpF | TTTGGATCCGGTTGTTGAAGACGCAG | apnT gene disruption |
| ApnTDownR | TCCTTTCCCATACTCTTC | apnT gene disruption |
| ApnTDownF | CAACTCGAGCGAGTTCTCTAAGTTA | apnT gene disruption |
| ApnTDownR | TTTGGTACCTGGTCAGAGACCGCATCT | apnT gene disruption |
| YbdGUpF | GGGACTAGTTCGAGATAG | ybdG gene disruption |
| YbdGUpR | CACCTGCAGATTATATACAGCGGAAG | ybdG gene disruption |
| YbdGDownF | TATCTCGAGGGTTCGCTAGGATGAAGG | ybdG gene disruption |
| YbdGDownR | TGAGGTACCGGTTTAAACATGCTGTC | ybdG gene disruption |
| ApnIUpF | GAAACTAGTTCTAGAGGCGGAACAAGAT | apnI gene disruption |
| ApnIDownR | GGGGAATTCATGAAAAGAACTGCGAACC | apnI gene disruption |
| ApnIDownF | TAACTCGAGAGGTTATACATATGGGC | apnI gene disruption |
| ApnIDownR | GCAACAAAGGTACCTGGTGAAATAG | apnI gene disruption |
| apnF | CCTGGATCCGCTCTGTTTCCGTTGTCG | Clone of apn cluster |
| apnR | TGAGGATCCTACCCAAGTGGTCTCATTATTT | Clone of apn cluster |
| ApnIF | TTCGGATCCGGTCAAGATTCGTGGGAC | Clone of fragment apnI-apnT (I) |
| ApnIR | GGCGAATTCGTTATTGCCCATATGTATAAC | Clone of fragment apnI |
| ApnTF | AGTGGATCCTAGCGAAAGTGTTCATTC | Clone of fragment apnT |
| ApnTR | GACGAATTCAAGCAAAACCAAAGCAGCC | Clone of fragment apnI-apnT (T) |
| ProapnAF | TTTGGATCCGCGAGCTGAAGTACAGTACG | Clone of the apnA promoter region |
| ProapnAR | CTTGGATCCTTCCATTTCCTCCTTTT | Clone of the apnA promoter region |
| ProapnAF2 | TTTGGATCCGCGAGCTGAAGTACAGTACG | Clone of the apnA promoter region |
| ProapnAR2 | CTTGAATTCCTTAGCTCGAGTTCCATTTCCTCCTTTT | Clone of the apnA promoter region |
| PropaeAF | AACGGATCCATCGGGACCAAAGTGATTCGACTCA | Clone of the paeA promoter region |
| PropaeAR | CTTGAATTCTGCCTCTCGAGTTTCCTCTTCCTCTCTGAATTAAAT | Clone of the paeA promoter region |
| PaeIF | TTTGGATCCCTTAGCTCGAGCAGATGCTGGTATTTAGCT | Clone of the paeI gene |
| PaeIR | CTCCGAATTCGAATGGGCCGTTTGTTTT | Clone of the paeI gene |
a
Restriction sites are underlined.