TABLE 2.
Plasmids used in this study
| Plasmid | Genotype | Reference |
|---|---|---|
| pYEX-BX | E. coli ori, 2μ, Ampr, leu2-d URA3 | 15 |
| pKL-BX | pYEX-BX with PGAL1::ORF4 | 15 |
| pKL-BX-C231A | pKL-BX with PGAL1::ORF4C231A | This work |
| pKL-BX-A231A | pKL-BX with PGAL1::ORF4A231C | This work |
| pGTIRUTIR | E. coli ori, TIR, URA3 from S. cerevisiae, TIR, Ampr | 33 |
| pGT-PO4PO2 | pGTIRUTIR with P. acaciae pPac1-2 ORF2 and ORF4 | This work |
| pGT-PO4PO2* | pGTIRUTIR with P. acaciae pPac1-2 ORF4::3HA and ORF2::3myc | This work |
| pBluescript SK(−) | ColE1ori, Ampr, lacZ | Stratagene, Heidelberg, Germany |
| pSK-RKF | pBluescript SK(−) with recombination flank k1ORF3/k1ORF4 | This work |
| pSK-LEU2* | pBluescript SK(−) with UCS::LEU2 from S. cerevisiae | This work |
| pSK-PADH1 | pBluescript SK(−) with S. cerevisiaei ADH1 promoter | This work |
| pSK-PO4PO2 | pBluescript SK(−) with P. acaciae pPac1-2 ORF2 and ORF4 | This work |
| pSK-PO4::HA-PO2::myc | pBluescript SK(−) with P. acaciae pPac1-2 ORF4::HA and ORF2::myc | This work |
| pSK-PO4PO2* | pBluescript SK(−) with P. acaciae pPac1-2 ORF4::3HA and ORF2::3myc | This work |
| pSK-PADH1::ORF2 | pBluescript SK(−) with PADH1::ORF2 | This work |
| pSK-PADH1::ORF4 | pBluescript SK(−) with PADH1::ORF4 | This work |
| YEplac195 | 2μ, URA3, Ampr, E. coli ori | 40 |
| p195-PADH1::ORF4 | YEplac195 with PADH1::ORF4 | This work |
| p195-PADH1::ORF4C231A | YEplac195 with PADH1::ORF4C231A | This work |
| p195-PADH1::ORF4A231C | YEplac195 with PADH1::ORF4A231C | This work |
| p195-PADH1::ORF2 | YEplac195 with PADH1::ORF2 | This work |
| p195-PADH1::ORF2D462A | YEplac195 with PADH1::ORF2D462A | This work |
| p195-PADH1::ORF2A462D | YEplac195 with PADH1::ORF2A462D | This work |
| p195-PADH1::ORF2E464A | YEplac195 with PADH1::ORF2E464A | This work |
| p195-PADH1::ORF2A464E | YEplac195 with PADH1::ORF2A464E | This work |
| p195-PADH1::ORF2E466A | YEplac195 with PADH1::ORF2E466A | This work |
| p195-PADH1::ORF2A466E | YEplac195 with PADH1::ORF2A466E | This work |
| p195-PADH1::ORF2D464–E466A | YEplac195 with PADH1::ORF2D464–E466A | This work |
| p195-PADH1::ORF2A464D-A466E | YEplac195 with PADH1::ORF2A464D-A466E | This work |
| p195-PADH1::ORF2C250A | YEplac195 with PADH1::ORF2C250A | This work |
| p195-PADH1::ORF2A250C | YEplac195 with PADH1::ORF2A250C | This work |
| pARS | ColE1 ori, Ampr, k1ORF4-LEU2*-k1ORF4 | This work |
| pARS-PO4PO2 | pARS with P. acaciae pPac1-2 ORF2 and ORF4 | This work |
| pAR3 | k1ORF2′-LEU2*-k1ORF2″, Ampr, E. coli ori | 32 |