FIG 1.

Candidate division TM7 bacterial sequences in dental plaque associated with orthodontic treatment. (A) DGGE profiles, obtained by PCR with TM7 primers, of DNA extracted from plaque collected at different time points from band and bond samples in a single subject. (B) Unrooted dendrogram of candidate division TM7 phylotypes identified following PCR from pooled plaque samples collected at different time points in panel A. The dendrogram shows clonal diversity of TM7 sequences within one patient. Phylogenetic analysis of clone sequences (N, shaded in gray) and database sequences (AF, AM, and AY numbers) indicated that a single individual carried at least 8 TM7 phylotypes orally. GenBank accession numbers for clones are as follows: N1, KM251184; N2, KM251185; N3, KM251186; N4, KM251187; N7, KM251188; N8, KM251189; N9, KM251190; and N12, KM251191.