FIG 1.

Functional analysis of PcGTT2.1. (A) Western blot confirming PcGTT2.1 overexpression in the yeast Δgtt1 Δgtt2 mutant. (B) Sensitivity of PcGTT2.1-complemented yeast strains to tert-butyl hydroperoxide (black bars) and hydrogen peroxide (gray bars). The growth of S. cerevisiae wild-type (wt; W303-1A) and Δgtt1 Δgtt2 mutant (MML1022) cells was automatically recorded as described in Materials and Methods. Exponential growth rates of treated cultures with the indicated agent concentrations, as well as control untreated cultures, were calculated, and the treated versus untreated ratios are represented. Bars correspond to the means from three independent experiments (plus standard deviations). (C) Measurement of 4-hydroxyalkenals (HAE) in Δgtt1 Δgtt2 mutants and in Δgtt1 Δgtt2 mutants complemented with PcGTT2.1. Bars correspond to the means from four independent experiments (plus standard deviations).