TABLE 5.
Kinetic parameters of PcGTT2.1 in H2O2, tBOOH, CuOOH, and HNE activity assays compared to those of other fungal peroxidase-like enzymesa
| Enzyme | Substrate |
Reference or source | |||||||
|---|---|---|---|---|---|---|---|---|---|
| H2O2 |
tBOOH |
CuOOH |
HNE |
||||||
| Km (mM) | Kcat (s−1) | Km (mM) | Kcat (s−1) | Km (mM) | Kcat (s−1) | Km (mM) | Kcat (s−1) | ||
| PcGTT2.1 | NA | NA | 0.55 ± 0.24 | 725.00 ± 119.90 | 0.011 ± 0.002 | 500.00 ± 13.52 | 0.15 ± 0.03 | 0.78 ± 0.07 | This study |
| ScGpx2 | 0.17 | 0.99 | 0.31 | 109.00 | ND | ND | ND | ND | 49 |
| ScUre2p | 4.30 | 0.36 | 7.90 | 0.07 | 7.80 | 0.37 | ND | ND | 50 |
| PcGSTFuA3 | NA | NA | NA | NA | 2.04 | 1.27 | ND | ND | 14 |
a
Experimental details are given in Materials and Methods. NA, not active; ND, not determined; Sc, S. cerevisiae; Pc, P. chrysosporium.