FIG 2.

qPCR validation of RNA-seq data for selected differentially expressed genes. The relative transcription of genes found to be differentially regulated in the RNA-seq analysis of the shock response (A) or the adaptive response (B) of S. Typhimurium to acidified NaNO₂ was examined by qPCR. 16S rRNA (A) or ampD (B) was used as a reference gene. Mean log₂ fold changes (FC) in the transcription of genes in four independent qPCR experiments were plotted against the respective log₂ FC determined by RNA-seq. The coefficient of determination (R²) was calculated in Microsoft Excel.