TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| WT | Salmonella enterica subsp. enterica serovar Typhimurium 14028; wild-type strain | DSM 19587 |
| WT pBR322 | WT strain carrying empty plasmid pBR322 | This study |
| ΔcadA pBR322 | Strain with in-frame deletion of the WT STM14_3138 DNA sequence encoding amino acids 7 to 703 of CadA (ΔcadA), carrying empty plasmid pBR322 | This study |
| ΔcadA-comp | ΔcadA strain carrying complementation plasmid pBR322-cadA | This study |
| WT pEGFP | WT strain carrying pEGFP for intracellular pH measurements | This study |
| Plasmids | ||
| pBR322 | pMB1 replicon cloning vector; Ampr Tetr | 24 |
| pBR322-cadA | Complementation plasmid containing the cadA coding sequence under the control of its native promoter, PcadBA; Ampr | This study |
| pEGFP | EGFP expression vector; Ampr | Clontech, Germany |
a
Ampr, ampicillin resistance; Tetr, tetracycline resistance.