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E. coli MG1655 harboring pTrc99A (A) or pTrc99A-Ptrc-budAB (B) grown on VRBG agar (top left), MacConkey agar containing 0.5% glucose (top right), EMB agar containing 0.5% glucose (bottom left), or LB agar containing 0.5% glucose and 20 mg/liter phenol red (bottom right) at 37°C for 24 h. All media contained 1 mM IPTG.
