FIG 4.
Difference in plate counts (expressed in log[CFU/ml]) between E. coli MG1655 lac::Tc/pTrc99A and E. coli MG1655 lac::Cm/pTrc99A-Ptrc-budAB during a 10-day cocultivation experiment with daily transfer to fresh medium. The medium was liquid LB (A) or LB agar (B) at an initial pH of 5.00 with 0.5% glucose, 1 mM IPTG, and 100 μg/ml ampicillin, and the growth temperature was 37°C. The lines shown represent the results of three independent replicate experiments. The plate counts of the two strains were significantly different from day 2 onward in both experiments.