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. 2014 Oct;80(19):6054–6061. doi: 10.1128/AEM.01711-14

FIG 5.

FIG 5

Mixed culture of E. coli MG1655 lac::Tc/pTrc99A (white arrows) and E. coli MG1655 lac::Cm/pTrc99A-Ptrc-budAB (black arrows), grown anaerobically on LB agar at pH 5.00 with 0.5% glucose, 1 mM IPTG, and 100 μg/ml ampicillin at 37°C for 24 h.