Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Strains and plasmids used in this study
| Strain or plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| MG1655 | F− rph-1 | 43 |
| MG1655 lac::Cm | Chloramphenicol resistance cassette in lacY | Laboratory collection |
| MG1655 lac::Tc | Tetracycline resistance cassette in lacZ | 44 |
| Plasmids | ||
| pTrc99A | Cloning vector carrying IPTG-inducible trc promoter (Ptrc); Apr | 15 |
| pTrc99A-Ptrc-budAB | pTrc99A carrying the budAB operon downstream of Ptrc; Apr | 6 |
