FIG 2.

Time course of 2C4NP degradation by Burkholderia sp. strain SJ98 in whole-cell biotransformation. In order to capture the intermediates before ring cleavage, 1 mM 2,2′-dipyridyl was added to inhibit the ring cleavage enzyme. Samples were withdrawn at the time points indicated and treated immediately as described in the text. The disappearance of 2C4NP and the appearance of the products (CBQ and CHQ) were quantified by HPLC. The experiments were performed in triplicate, the results shown are average values of three independent experiments, and error bars indicate standard deviations.