FIG 4.

Time course of 2C4NP degradation and cell growth in cultures of Burkholderia sp. strains SJ98, SJ98ΔpnpA (with pnpA deleted), and SJ98ΔpnpA1 (with pnpA1 deleted). The data for pnpA-complemented mutant SJ98ΔpnpA(pRK415-pnpA) are not shown. The results obtained with PNP were similar to those obtained with 2C4NP (data not shown). N, number of cells; N₀, initial number of cells. The growth curves were fitted by the modified Gompertz equation (33) with OriginPro (version 8) software. All of the experiments were performed in triplicate, the results shown are average values of three independent experiments, and error bars indicate standard deviations.