TABLE 2.
Comparison of 16SrRNA-specific qPCR data, amoA-specific qPCR data, and FISH data with dilution factors for the serial dilution samplesa
| Model parameter | Estimate | SE | P | 95% CI (maximum, minimum) |
|---|---|---|---|---|
| First dilution | ||||
| 16S rRNA-specific qPCR | ||||
| Intercept | 8.0 | 0.078 | 0.000*** | 7.8, 8.2 |
| Coefficient of log qPCR 16S | 0.92 | 0.029 | 0.000*** | 0.86, 0.98 |
| amoA-specific qPCR | ||||
| Intercept | 9.8 | 0.14 | 0.000*** | 9.5, 10 |
| Coefficient of log qPCR amoA | 1.4 | 0.077 | 0.000*** | 1.3, 1.6 |
| FISH | ||||
| Intercept | 8.8 | 0.097 | 0.000*** | 8.6, 9.0 |
| Coefficient of log FISH | 1.1 | 0.036 | 0.000*** | 0.98, 1.1 |
| Second dilution | ||||
| 16S rRNA-specific qPCR | ||||
| Intercept | 6.4 | 0.079 | 0.000*** | 6.2, 6.5 |
| Coefficient of log qPCR 16S | 0.92 | 0.029 | 0.000*** | 0.86, 0.98 |
| amoA-specific qPCR | ||||
| Intercept | 8.4 | 0.18 | 0.000*** | 8.0, 8.8 |
| Coefficient of log qPCR amoA | 0.92 | 0.089 | 0.000*** | 0.75, 1.1 |
| FISH | ||||
| Intercept | 8.2 | 0.098 | 0.000*** | 8.0, 8.4 |
| Coefficient of log FISH | 1.1 | 0.036 | 0.000*** | 0.98, 1.1 |
a
Coefficients and corresponding standard errors, P values, and 95% confidence intervals (CI) for the model parameters of a comparison of 16S rRNA-specific qPCR data, amoA-specific qPCR data, and FISH data with dilution factors for the serial dilution samples are shown. ***, highly significant P value.