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. 2014 Oct;80(19):5901–5910. doi: 10.1128/AEM.01383-14

TABLE 4.

Comparison of 16S rRNA-specific qPCR data with amoA-specific qPCR data, 16S rRNA-specific qPCR data with FISH data, and amoA-specific qPCR data with FISH data for the time-series samplesa

Model parameter Estimate SE P 95% CI (maximum, minimum)
Comparison of 16SrRNA-specific qPCR data with the amoA-specific qPCR
    Intercept 2.3 0.47 0.000*** 1.4, 3.2
    Coefficient of log qPCR amoA 0.46 0.075 0.000*** 0.31, 0.61
Comparison of the 16S rRNA-specific qPCR data with the FISH data
    Intercept 2.2 0.45 0.001** 1.3, 3.1
    Coefficient of log FISH 0.43 0.064 0.000*** 0.30, 0.56
Comparison of amoA-specific qPCR data with the FISH data
    Intercept 0.60 0.59 0.794 −0.56, 1.8
    Coefficient of log FISH 0.81 0.097 0.000*** 0.62, 1.0
a

Coefficients and corresponding standard errors, P values, and 95% confidence intervals (CI) for the model parameters of the comparison of the 16S rRNA-specific qPCR data with the amoA-specific qPCR data, the 16S rRNA-specific qPCR data with the FISH data, and the amoA-specific qPCR data with the FISH data for the time-series samples are shown. **, very significant P value; ***, highly significant P value.