TABLE 5.

Model parameters for mixed-effects models for comparisons between the two qPCR datasets and between each qPCR and FISH for the entire data set^a

Model parameter	Estimate	SE	P	95% CI (maximum, minimum)
16S rRNA vs amoA qPCR
Intercept for first dilution	1.4	0.50	0.012	0.42, 2.4
Intercept for second dilution	–1.8	0.47	0.001**	−2.8, −0.92
Intercept for full-scale and lab-scale	2.3	0.55	0.001**	1.2, 3.4
Log amoA for first dilution	0.68	0.050	0.001**	0.59, 0.79
Log amoA for second dilution	0.97	0.040	0.000***	0.89, 1.0
Log amoA for full-scale and lab-scale	0.46	0.090	0.000***	0.28, 0.64
SD u(group)	0.40			0.27, 0.59
SD ε	0.13			0.082, 0.21
16S rRNA qPCR vs FISH
Intercept for first dilution	0.43	0.45	0.35	0.22, 2.1
Intercept for second dilution	–0.74	0.42	0.096	−0.45, 1.3
Intercept for full-scale	–1.4	0.34	0.001**	−2.0, −0.69
Intercept for lab-scale	–0.15	0.26	0.57	−0.66, 0.36
Log FISH	0.86	0.040	0.000***	0.78, 0.94
SD u(group)	0.34			0.21, 0.54
SD ε	0.19			0.12, 0.29
amoA qPCR vs FISH
Intercept for first and second dilution	0.53	0.54	0.34	−0.53, 1.6
Intercept for full-scale	–0.76	0.60	0.22	−1.9, 0.42
Intercept for lab-scale	–0.33	0.43	0.46	−1.2, 0.51
Log FISH	0.98	0.070	0.000***	0.84, 1.1
SD u(group)	0.31			0.098, 0.98
SD ε	0.36			0.21, 0.59

^aCoefficients and corresponding standard errors, P values, and 95% confidence intervals (CI) for the model parameters for the mixed-effects models for the comparisons between the two qPCR datasets and between each qPCR and FISH for the entire data set are shown. **, very significant P value; ***, highly significant P value.