TABLE 2.
Primer or probe function and name | DNA sequence (5′–3′) | Reference or source |
---|---|---|
Identification of leaves positive for “Ca. Liberibacter asiaticus” | ||
OI1 | GCG CGT ATG CAA TAC GAG CGG CA | 15 |
OI2c | GCC TCG CGA CTT CGC AAC CCA T | 15 |
CG03F | RGG GAA AGA TTT TAT TGG AG | 16 |
CG05R | GAA AAT AYC ATC TCT GAT ATC GT | 16 |
RT-qPCR | ||
SC1_gp025F | AGC TAG ATC ATT GAC TCT TCC | This work |
SC1_gp025R | AAA GAT GTT GGT CGT AAA CTA G | This work |
SC2_gp095F | GGT GAA TAG CTA GAA TCC CG | This work |
SC2_gp095R2 | TGT TGC TGG AGC CTA TAA CG | This work |
SC2_gp100F | GTT CCA ATT CTC TAT AAG CGG | This work |
SC2_gp100R2 | CGA ATG ATC CGA TTG CTT TAG | This work |
SC1_gp110F2 | TCG TAC ATG CAC CCC TGA TA | This work |
SC1_gp110R2 | AAG TGA GAC GCC AGG AAA GT | This work |
LasprfAF | TGT CTG AAT CGC CTT CTG TC | This work |
LasprfAR | GAT CAC CGA TGA CAG TAT GC | This work |
LasgyrBF | TTG AAC AAG CTG TAA TTT CTG G | This work |
LasgyrBR | ATC TGT TTG CCA ATT TAG AAG C | This work |
SC1_gp025P | 6-FAM-ATA TTC ATC-ZEN-TTT TGT GTT AAA ACG GC-IABkFQa | This work |
SC2_gp095P | 6-FAM-TGT TAG AGA-ZEN-AAC TAT CAC GTT CAA C-IABkFQ | This work |
SC2_gp100P | 6-FAM-TCA AAT CCG-ZEN-TAA TAT AAA GGG CTA TT-IABkFQ | This work |
SC1_gp110P | 6-FAM-TAT TCT ATT-ZEN-CAT CTC GTA AGC ACG T-IABkFQ | This work |
LasprfAP | 6-FAM-TCA GTC CTA-ZEN-TAA TAT CGA AGA TTA GT-IABkFQ | This work |
LasgyrBP | 6-FAM-TTA CAT TAA-ZEN-TTT CTT GAT CAC TTT CAC-IABkFQ | This work |
Functional analysis of potential lysis genes | ||
CLas endolysin NdeI F | TTA CAT ATG GTG TGT ATT ATA AAC AGA ATA ATA T | This work |
CLas endolysin NheI R | TTA GCT AGC ATC ATA TCT ACA ATC CTC TTC TC | This work |
SC1_gp035 NdeI F | TAT CAT ATG TAT TTC AAC GCG GTA AGC | This work |
SC1_gp035 NheI R | AAT GCT AGC TCA TAA AGA TCC TCC TGT TCG | This work |
Holin NdeI F | TTA CAT ATG AGT TTC TTA GAT TCG AGT G | This work |
Holin NdeI F2 | TTA CAT ATG GGC GGA GCA GCA GGT | This work |
Holin NheI R | AAT GCT AGC GAT CTT TGA TTC ATC GTA CAT G | This work |
Promoter expression reporter constructs | ||
HPromRIF | CUA CUA CUA CUA CCT AGG CCG ATA AAC TCC AAA AAA CGA G | This work |
HPromFIR1 | GAU GAU GAU GAU CGT ACG TGA CGC AAA TAA CAC TGG TGC | This work |
HPromFIR2 | GAU GAU GAU GAU CGT ACG AGT TTG CGA GCC TTA TCA ACC | This work |
HPromFIR3 | GAU GAU GAU GAU CGT ACG CGG GCT TAT GTA TAC CTT TGC | This work |
HPromFIR4 | GAU GAU GAU GAU CGT ACG GCG GAG GTT TGA ATA TGA CTC | This work |
pUC19VF2 | UAG UAG UAG UAG GAG ACA GCT ATG ACC ATG ATT AC | This work |
pUC19VR | AUC AUC AUC AUC CGG TTT GCG TAT TGG GCG C | This work |
pGUSIR | GAU GAU GAU GAU CGT ACG ATA CCT AGG AGT CCC TTA TGT TAC GTC CTG | This work |
pGUS IR2 | TTA GGA TCC AGG AGT CCC TTA TGT TAC GTC CTG | This work |
pGUS IF2 | TTA AAG CTT CAG GAG AGT TGT TGA TTC ATT G | This work |
IABkFQ, Iowa Black FQ quencher.