TABLE 2.

Primers and probes used in this study

Primer or probe function and name	DNA sequence (5′–3′)	Reference or source
Identification of leaves positive for “Ca. Liberibacter asiaticus”
OI1	GCG CGT ATG CAA TAC GAG CGG CA	15
OI2c	GCC TCG CGA CTT CGC AAC CCA T	15
CG03F	RGG GAA AGA TTT TAT TGG AG	16
CG05R	GAA AAT AYC ATC TCT GAT ATC GT	16
RT-qPCR
SC1_gp025F	AGC TAG ATC ATT GAC TCT TCC	This work
SC1_gp025R	AAA GAT GTT GGT CGT AAA CTA G	This work
SC2_gp095F	GGT GAA TAG CTA GAA TCC CG	This work
SC2_gp095R2	TGT TGC TGG AGC CTA TAA CG	This work
SC2_gp100F	GTT CCA ATT CTC TAT AAG CGG	This work
SC2_gp100R2	CGA ATG ATC CGA TTG CTT TAG	This work
SC1_gp110F2	TCG TAC ATG CAC CCC TGA TA	This work
SC1_gp110R2	AAG TGA GAC GCC AGG AAA GT	This work
LasprfAF	TGT CTG AAT CGC CTT CTG TC	This work
LasprfAR	GAT CAC CGA TGA CAG TAT GC	This work
LasgyrBF	TTG AAC AAG CTG TAA TTT CTG G	This work
LasgyrBR	ATC TGT TTG CCA ATT TAG AAG C	This work
SC1_gp025P	6-FAM-ATA TTC ATC-ZEN-TTT TGT GTT AAA ACG GC-IABkFQa	This work
SC2_gp095P	6-FAM-TGT TAG AGA-ZEN-AAC TAT CAC GTT CAA C-IABkFQ	This work
SC2_gp100P	6-FAM-TCA AAT CCG-ZEN-TAA TAT AAA GGG CTA TT-IABkFQ	This work
SC1_gp110P	6-FAM-TAT TCT ATT-ZEN-CAT CTC GTA AGC ACG T-IABkFQ	This work
LasprfAP	6-FAM-TCA GTC CTA-ZEN-TAA TAT CGA AGA TTA GT-IABkFQ	This work
LasgyrBP	6-FAM-TTA CAT TAA-ZEN-TTT CTT GAT CAC TTT CAC-IABkFQ	This work
Functional analysis of potential lysis genes
CLas endolysin NdeI F	TTA CAT ATG GTG TGT ATT ATA AAC AGA ATA ATA T	This work
CLas endolysin NheI R	TTA GCT AGC ATC ATA TCT ACA ATC CTC TTC TC	This work
SC1_gp035 NdeI F	TAT CAT ATG TAT TTC AAC GCG GTA AGC	This work
SC1_gp035 NheI R	AAT GCT AGC TCA TAA AGA TCC TCC TGT TCG	This work
Holin NdeI F	TTA CAT ATG AGT TTC TTA GAT TCG AGT G	This work
Holin NdeI F2	TTA CAT ATG GGC GGA GCA GCA GGT	This work
Holin NheI R	AAT GCT AGC GAT CTT TGA TTC ATC GTA CAT G	This work
Promoter expression reporter constructs
HPromRIF	CUA CUA CUA CUA CCT AGG CCG ATA AAC TCC AAA AAA CGA G	This work
HPromFIR1	GAU GAU GAU GAU CGT ACG TGA CGC AAA TAA CAC TGG TGC	This work
HPromFIR2	GAU GAU GAU GAU CGT ACG AGT TTG CGA GCC TTA TCA ACC	This work
HPromFIR3	GAU GAU GAU GAU CGT ACG CGG GCT TAT GTA TAC CTT TGC	This work
HPromFIR4	GAU GAU GAU GAU CGT ACG GCG GAG GTT TGA ATA TGA CTC	This work
pUC19VF2	UAG UAG UAG UAG GAG ACA GCT ATG ACC ATG ATT AC	This work
pUC19VR	AUC AUC AUC AUC CGG TTT GCG TAT TGG GCG C	This work
pGUSIR	GAU GAU GAU GAU CGT ACG ATA CCT AGG AGT CCC TTA TGT TAC GTC CTG	This work
pGUS IR2	TTA GGA TCC AGG AGT CCC TTA TGT TAC GTC CTG	This work
pGUS IF2	TTA AAG CTT CAG GAG AGT TGT TGA TTC ATT G	This work

^aIABkFQ, Iowa Black FQ quencher.