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Growth curve (A) and internal pH (B) of B. subtilis PB2 pDG-IpHluorin monitored for 6 h under various stress conditions. The medium pH was 6.4. In M3G, the growth rate is the highest, but a continuous decrease in pHin is seen. Sorbic acid stress causes the growth rate to be reduced, as well as a continuous decrease in internal pH. Acetic acid stress has a similar effect on the growth rate, but pHin remains constant.
