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Acidification and recovery upon weak organic acid addition to B. subtilis PB2 PptsG-IpHluorin. Sorbic acid (A and B) or acetic acid (C and D) was injected at time zero. The medium pH was 6.4. The internal pH drops to its lowest point within seconds (A and C) and recovers fast when glucose is available (B and D). The pH recovers to higher values with acetic acid stress. Data from typical examples are shown.
