FIG 3.
Heptadecanoic acid (C17) and 1-octadecene (C18:1) formation after the addition of pentadecanoic acid (C15). The formation of C17 and C18:1 was observed for the wild-type (wt) and Δaa1977 strains but not for the Δaas675 strain. Peak areas obtained from GC-MS analysis were normalized to the volume, OD730, and the area of an internal standard; they are presented as a fraction of the wild-type-normalized area for the corresponding compound. Error bars represent the standard deviations from three biological replicates. n.d., not detected.