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Kinetic parameters of TcTS with 4MU-Neu5Ac as donor substrate and β3′-GL, β4′-GL, β6′-GL, and lactose as acceptor substratesa
| Acceptor substrate | Km (mM) | Vmax (μmol · min−1 · mg−1) | kcat (s−1) | kcat/Km (mM−1 · s−1) |
|---|---|---|---|---|
| 3′-Galactosyl-lactose | 0.06 | 13.9 | 3.7 | 62.0 |
| 4′-Galactosyl-lactose | 0.32 | 14.0 | 3.4 | 10.5 |
| 6′-Galactosyl-lactose | 0.13 | 12.5 | 3.0 | 23.3 |
| Lactose | 0.79 | 22.9 | 4.8 | 6.1 |
The data represent averages from triplicate experiments (for details, see Materials and Methods).
