TABLE 3.
Genes, primers, and amplicons used for qPCR analysis
| Genea | Primerb | Amplicon size (bp) |
|---|---|---|
| luxS | F, 5′-ATGAGCAGCGTGTTGCTGATGC-3′ | 122 |
| R, 5′-CAACGAGTGCATCTGGTAAGTGC-3′ | ||
| fliC | F, 5′-ATTAACAGCGCGAAGGATGACG-3′ | 180 |
| R, 5′-TACCGTCAGTTCACGCACACG-3′ | ||
| csgA | F, 5′-GATCTGACCCAACGTGGCTTCG-3′ | 178 |
| R, 5′-GATGAGCGGTCGCGTTGTTACC-3′ | ||
| fimA | F, 5′-CTCTGGCAATCGTTGTTCTGTCG-3′ | 119 |
| R, 5′-GCAAGCGGCGTTAACAACTTCC-3′ | ||
| gapA* | F, 5′-GATTACATGGCATACCTG-3′ | 244 |
| R, 5′-CAGACGAACGTTCAGGTCAA-3′ |
a
luxS, AI-2 synthase gene; fliC, flagellin gene; csgA, major curli subunit gene; fimA, major type 1 subunit fimbrin gene; gapA*, glyceraldehyde-3-phosphate dehydrogenase gene (internal control for qPCR).
b
F, forward; R, reverse.