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Syncytium formation with gB843 is enhanced over that with wt gB. (A and B) CHO-K1 cells were transfected with wt gB or gB843 along with gH/gL and were overlaid with HEK293 cells. Syncytium formation was visualized by surface staining of the cells with antibody E1D1 against the gH/gL complex. (C) The areas of the individual syncytia were quantified using ImageJ. The asterisk indicates a significant difference (P < 0.05) by the Student t test.
