FIG 4.

Association of gB and gB843 with gH/gL. CHO-K1 cells were transfected with EBV gB or gB843 together with Flag-gH/gL. After 18 h of transfection, CHO-K1 cells were seeded in 16-well chamber slides. The chamber slides were then fixed with methanol after 24 h of culture. The proximity of gB to gH/gL was examined using a Duolink kit (Olink) with monoclonal antibody CL55 and the rabbit anti-Flag antibody from Sigma (center), and nuclei were stained with DAPI (bottom). gB was further stained using the Alexa Fluor 488-conjugated anti-mouse secondary antibody (Invitrogen) after Duolink antibodies were added (top).