FIG 8.

Time and temperature dependence of fusion with gB843. (A) CHO-K1 cells were transiently transfected with the T7 luciferase plasmid and a vector plasmid (control) or with the T7 luciferase plasmid, gH/gL, and EBV gB or gB843, as indicated. Transfected CHO-K1 cells were overlaid with epithelial cells at different temperatures, as indicated, for 24 h, and luciferase activity was measured. The data are means plus standard errors of the means for three independent experiments. (B) CHO-K1 cells were transiently transfected with the T7 luciferase plasmid and a vector plasmid (control) or with the T7 luciferase plasmid, gH/gL, and EBV gB or gB843, as indicated. Transfected CHO-K1 cells were overlaid with epithelial cells, and luciferase activity was measured after different incubation times, as indicated. The data are means plus standard errors of the means for three independent experiments.