FIG 6.

Ribosome binding to the 3′TSS. Filter-binding assays were conducted using three different-sized fragments that contain the 3′TSS (S1, S2, and S3). (A) Locations of the endpoints of the fragments used for filter binding. (B) One to 60 pmol of [³²P] 5′-end-labeled fragments were combined with 15 pmol of salt-washed ribosomes or separated subunits purified from Arabidopsis protoplasts. The standard deviations are shown for three experiments. The values for the TCV TSS (31) and PEMV kl-TSS (13) were previously published and are presented here for comparison.