FIG 7.

3′TSS and kl-H function as 3′CITEs in WGE when proximal to the luciferase reporter termination codon. (A) Location of mutations in 3′TSS, kl-H, and 5′89. The putative kissing-loop interaction between hairpin 5H2 in the 5′89 nt of PEMV and the kl-H is shown. Sequences shared between the kl-H and hairpin 3H1 of the kl-TSS (which is known to interact with 5H2) are boxed. (B) Relative luciferase activity in WGE programmed with 5′89+3U containing mutations in the kl-H and 3′TSS. Asterisks denote data previously published (13) and presented here for comparison. The data are from three independent experiments performed in triplicate, and the standard deviations are shown. (C) Diagram of 5′89+3Umini. This construct contains a deletion that removes the kl-TSS and PTE and places the kl-H just downstream of the luciferase ORF. (D) Relative luciferase activity of 5′89+3Umini and 5′89+3Umini that contains mutations in the kl-H and 3′TSS shown in panel A.