FIG 8.

3′TSS and kl-H function as 3′CITEs in protoplasts when proximal to the luciferase reporter termination codon. (A) Relative luciferase activity in protoplasts at 18 h after transformation with 5′89+3U transcripts containing mutations in the kl-H and 3′TSS that were described in Fig. 7A. Asterisks denote data previously published (13) and presented here for comparison. The data are from three independent experiments performed in triplicate, and the standard deviations are shown. (B) Relative luciferase activity in protoplasts of 5′89+3Umini and 5′89+3Umini containing mutations in the kl-H and 3′TSS.