FIG 5.

AGO-loaded vd-sRNAs adopt hot spot distributions along the viroid genome that are specific for each of the four HA-tagged AGOs from A. thaliana agroexpressed in PSTVd-infected N. benthamiana. Shown are the locations and frequencies in the genomic PSTVd RNA of the 5′ termini of the plus-strand (positive values) and minus-strand (negative values) vd-sRNA reads per million (rpm) from total RNAs (Input) and from immunoprecìpitates (IP) generated with an anti-HA monoclonal antibody. Note that the same numbers are used in the plus polarity (the 5′-to-3′ orientation is from left to right) and in the minus polarity (the 5′-to-3′ orientation is from right to left).