FIG 2.

De novo infection of C. elegans by virus produced from transgenic animals. (A) Real-time qRT-PCR quantification of both recombinant wild-type Orsay virus (rec-WT) and naturally occurring Orsay virus (nat-WT) replication in C. elegans N2 and rde-1 strains. A nontransgenic C. elegans N2 preparation was used as a mock control. N/D, viral RNA was not detected. Error bars indicate standard deviations from three independent infection experiments. (B) Western blot with anti-Orsay virus capsid antibody. Arrow indicates the virus capsid band. (C) Immunofluorescence staining of recombinant and natural Orsay virus replication in infected C. elegans with anti-Orsay virus capsid antibody. Arrows indicate the infected intestinal cells. Scale bars represent 20 μm.