FIG 4.

Generation and verification of a FLAG epitope-tagged Orsay virus. (A) A schematic design of FLAG-tagged Orsay virus RNA2 genome segment. Both the genome construct and expected proteins are shown. The nucleotide and amino acid sequences of FLAG peptide tag are underlined, and those for the linker peptide are italicized. #, stop codon. (B) RT-PCR and direct PCR were performed on the FLAG-tagged virus-infected C. elegans. (C) Double immunofluorescence staining of rec-WT-infected C. elegans with anti-Orsay capsid and anti-Orsay delta peptide antibodies. Scale bars represent 10 μm. (D) Double immunofluorescence staining of rec-FLAG- and rec-WT-infected C. elegans with anti-Orsay capsid peptide antibody and monoclonal anti-FLAG antibody. (E) Double immunofluorescence staining of rec-FLAG- and rec-WT-infected C. elegans with anti-Orsay delta peptide antibody and monoclonal anti-FLAG antibody.