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Formation of PrPSc-seeded rPrP fibrils in the 2nd round (2nd-rPrP-fibSc) and 5th round (5th-rPrP-fibSc) of RT-QUIC. (A) Between each round, the reaction mixtures from the previous reaction were diluted 100-fold into fresh rPrP. The reaction buffer contained 300 mM NaCl, 50 mM HEPES (pH 7.5), and 10 μM ThT. The rPrP concentration was 100 μg/ml. (B) TEM analysis of PrPSc-seeded rPrP fibrils generated in the second and fifth rounds of RT-QUIC. Bars, 100 nm.
