FIG 3.
Silver staining and Western blot analysis of purified PrPSc. (A) The purified PrPSc samples (P) were examined by silver-stained SDS-polyacrylamide gel analysis (left). For comparison, the electrophoretic pattern of prion-infected BHs containing 100 μg total protein digested with PK (20 μg/ml, 37°C for 1 h) is shown (left). The purified PrPSc samples were immunoblotted with polyclonal anti-PrP antibody M20 (right). Molecular mass markers (lane M) are indicated in kilodaltons (kDa) on the left side of each panel. (B) Electron microscopy analysis of purified 22L PrPSc (left) and Chandler PrPSc (right). Bars, 100 nm.