FIG 1.

(A) Analysis of the ectopic expression of HRV16 nonstructural proteins by Western blotting. HeLa cells were transfected with the indicated pRK5-Myc constructs or the empty vector (−). Cells then were lysed in Laemmli sample buffer 24 h posttransfection, and the DNA content of the samples was assessed by NanoDrop measurement. The same amount of DNA was loaded for each sample, and the proteins were revealed by Western blotting with an anti-Myc antibody. The position of the proteins is indicated on the right, together with their predicted molecular mass in kDa. (B) HRV16 3A and 3AB are expressed at higher levels from transfected cells than from infected cells. HeLa Ohio cells were infected for 24 h with HRV16 at the indicated MOI, transfected with cDNA constructs allowing the expression of N-terminal Myc tagged HRV16 3A or 3AB, or left untreated (cells). Cells then were lysed in Laemmli sample buffer, and the DNA content of the samples was assessed by NanoDrop measurement. The same amount of DNA was loaded for each sample, and the 3A protein and β-actin loading control were revealed by Western blotting with anti-3A and anti-β-actin antibodies, respectively.