FIG 3.

Effect of rhinovirus HRV16 nonstructural protein expression on the endoplasmic reticulum. HeLa Ohio cells were transfected with cDNA constructs allowing the expression of the indicated HRV16 nonstructural proteins or the GFP control with an N-terminal Myc tag. The nonstructural proteins and the endoplasmic reticulum marker calnexin were stained by immunofluorescence with anti-Myc and anti-calnexin antibodies, respectively, revealing partial colocalization of the nonstructural proteins 2C, 3A, and 3AB with calnexin and redistribution of the endoplasmic reticulum marker to 2B- and 2BC-containing aggregates. Images are single confocal 0.37-μm optical sections. Scale bar, 10 μm.