FIG 5.

Transfected HRV16 3A and 3AB significantly disrupt the Golgi structure. HeLa Ohio cells were transfected with cDNA constructs allowing the expression of the indicated HRV16 nonstructural proteins or the GFP control with an N-terminal Myc tag. The nonstructural proteins and the Golgi marker Giantin were stained by immunofluorescence with anti-Myc and anti-Giantin antibodies, respectively. For each condition, the Golgi structure of 100 transfected cells was assessed and classified in one of the three categories depicted in panel A: compact, extended, and dispersed. For control vector-transfected cells, 100 random cells were evaluated. The mean number of cells in each category from 3 independent experiments is represented in panel B. Error bars represent the standard errors of the means (SEM). Results were analyzed with GraphPad Prism 6 software, using a two-way ANOVA followed by Dunnett's multiple-comparison posttest. Significant differences compared to the GFP-transfected control are marked.