FIG 6.

Transfected HRV16 2B and 3A significantly disrupt secretion of the Gaussia luciferase reporter. HeLa Ohio cells were cotransfected with constructs allowing the expression of the naturally secreted Gaussia luciferase (Gluc) and the indicated HRV16 nonstructural proteins or the GFP control with an N-terminal Myc tag. After 24 h of transfection, the cell medium was replaced and the Gluc was allowed to be secreted for 2 h. The Gluc activity then was measured in the medium and cell lysates, allowing the calculation of the percentage of secreted Gluc, which represents the Gluc activity in the media divided by the total Gluc activity present in the media and lysates combined. The data represent the mean percentages of Gluc secreted from 3 independent experiments. Error bars represent the standard errors of the means (SEM). Results were analyzed with GraphPad Prism 6 software, using one-way ANOVA followed by Dunnett's multiple-comparison posttest. Significant differences compared to the GFP-transfected control are marked.