FIG 7.

Effect of HRV16 infection on the cis-Golgi structure. HeLa Ohio cells were uninfected or infected with HRV16 at an MOI of 20 for the indicated periods of time. The nonstructural protein 2C and the Golgi marker GM130 were stained by immunofluorescence, revealing Golgi structure disruption in infected (2C-positive) cells compared to the compact Golgi structure observed in uninfected cells. As a control, HeLa cells were left untreated or treated with BFA to disrupt the Golgi structure. Images are single confocal 0.37-μm optical sections. Scale bar, 10 μm.