FIG 9.

HRV16 infection significantly disrupts the Golgi structure. HeLa Ohio cells were uninfected or infected with HRV16 at the indicated multiplicity of infection (MOI) and for the indicated periods of time. Treatment with BFA was used as a control. The nonstructural protein 2C and the Golgi marker GM130 were stained by immunofluorescence. At a time point when infected cells could be detected by 2C staining, the Golgi structure of 100 infected (2C-positive) cells was assessed and classified in one of three categories, as depicted in Fig. 5A. Otherwise (for uninfected cells or cell infected for 2 h), 100 random cells were evaluated. Results represent the mean number of cells in each category from 3 independent experiments. Error bars represent the standard errors of the means (SEM). Results were analyzed with GraphPad Prism 6 software, using a two-way ANOVA followed by Dunnett's multiple-comparison posttest. Significant differences compared to the uninfected control are marked.