FIG 4.

Expression of M74-G in cells and on the cell surface. (A) Transfected cells expressing M74-G-FLAG were lysed and subjected to SDS-PAGE under reducing conditions and Western blotting. G proteins were immunostained by antibodies directed against the FLAG tag. Exposure time of 35 s. (B) For detection of G protein on the cell surface, transfected cells were subjected to surface biotinylation. The biotinylated proteins were separated with streptavidin-agarose and analyzed by SDS-PAGE and Western blotting. G proteins were detected by immunostaining. Exposure time of 120 s. Molecular mass markers are indicated on the left (kDa).