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. 2014 Oct;88(20):11973–11980. doi: 10.1128/JVI.01561-14

FIG 5.

FIG 5

Flow cytometry analysis of the surface expression of M74-G. Transfected cells expressing no foreign protein (pCG1) or FLAG-tagged NiV-G or M74-G were incubated with an antibody against the FLAG epitope, followed by incubation with anti-mouse biotin and streptavidin phycoerythrin. The amount of surface-expressing cells was analyzed by flow cytometry. (A) Normalized surface expression of M74-G. To compare the results obtained for the M74-G, the surface expression was normalized to the surface expression of NiV-G, which was set as 100%. The normalized M74-G surface expression is given in percentage of positive cells. Asterisks indicate significance (*, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001). (B) Histogram plots of NiV-G and M74-G surface expression in chiropteran and nonchiropteran cells. Black curves represent the histogram plot of pCG1-transfected cells; green and red curves indicate the histogram plots of NiV-G and M74-G surface expression, respectively. The gate setting is indicated by the black line.