Skip to main content
. 2014 Oct;88(20):11973–11980. doi: 10.1128/JVI.01561-14

FIG 8.

FIG 8

Expression of chimeric G proteins of NiV and M74. (A) HypNi/1.1 cells were transfected for coexpression of NiV-G-HA (green) with either M74-G ED or NiV-G ED (red). Permeabilized cells were immunostained for NiV-G with antibodies against the HA tag (green) and for chimeric proteins with antibodies directed against the FLAG-tag (red). (B) Cells were transfected for expression of the FLAG-tagged native G proteins, NiV-G or M74-G, or either of the corresponding ED chimeras, NiV-G-FLAG ED or M74-G-FLAG ED. The nonpermeabilized cells (−TX) were immunostained for the presence of G proteins by antibodies directed against the FLAG tag. Scale bars indicate 25 μm.