FIG 6.

Evaluation of PLpro inhibitor 3e in the SARS-CoV MA15 mouse model. (A) Five 8-week-old BALB/c mice from each group were administered either a single dose of 20 μg poly(I·C) (Poly IC) i.n. or 50 μg per dose of 3e or vehicle i.p. at 4 h prior to infection i.n. with 2 × 10⁴ PFU strain MA15 virus. The infected mice were further treated with 3e or vehicle twice a day at 0, 1, and 2 days p.i. The mice were monitored for weight loss and mortality. (B) The viral titer in the lung was determined at 4 days p.i. The statistical differences in weight loss and titer were analyzed with Prism software using the t test. The error bars represent SEM.