FIG 3.

Immunofluorescence analysis of VEEV-GP, EEEV-GP, and WEEV-GP replicon electroporated Vero cells. Vero cells were electroporated with VEEV-GP, EEEV-GP, or WEEV-GP RNA. Following electroporation, cells were seeded in a 48-well plate. At 18 h postelectroporation, cells were fixed with acetone-methanol (1:1), and IFA was performed. E2 protein was detected using anti-VEEV, anti-EEEV, or anti-WEEV E2-specific monoclonal antibodies and visualized by Alexa Fluor 288-labeled secondary antibody. Control cells received no RNA at electroporation and show no specific fluorescence with any of the antibodies in the assay. α, anti.