FIG 6.

Provision of additional PR8-HA-specific CD4⁺ T cells augments virus-specific Ab responses but does not alter the skewed GC/ASC response in bacterial coinfection. (A and B) MedLN from mice with or without adoptively transferred naive CD4⁺ T cells from TS1 mice were analyzed at day 10 after PR8 infection for PR8-specific and total IgG ASCs by ELISPOT assay (A) and the percentage of PR8 HA-specific GC B cells and CD138⁺ ASCs by cell surface staining with the PR8 HA probe (B). (C) Numbers of PR8 HA-specific GC B cells and PR8-specfic IgG ASCs in the medLN from indicated groups. (D) GC B cells and CD138⁺ ASCs in medLN from single PR8- and coinfected mice in the adoptive transfer model at indicated days after PR8 infection. (E and F) PR8-specific C12Id⁺ Ig (E) and IgM and IgG (F) Ab in serum of mice with or without adoptively transferred CD4⁺ T cells from TS1 mice (day 10 p.i. with PR8). (G) Total IgG serum Abs in indicated groups (days 34 and 35 p.i. with PR8). At least n = 5 mice/group. Significance was determined by Student's t test. ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.